Tingkat Prevalensi Parasit Perkinsus sp. terhadap kerang di Teluk Jakarta
Abstract
Perkinsiosisis a disease caused by the protozoan parasite Perkinsus sp. that can infect shellfish, especially bivalve molluscs, in various parts of the world. Molecular identification and prevalence of Perkinsus sp. parasitism in shellfish traded in Jakarta, especially green mussel (Perna viridis), blood cockle (Anadara granosa) and blood clam (Anadara inflata)were collected from TPI Muara Angke, TPI Muara Kamal, TPI Muara Baru and TPI Cilincing. Established from gill and mantle fragment examined with Polymerase Chain Reaction (PCR) test. The primer of Perkinsus genus is PerkITS‐85 forward 5 'CCG GAT TTG CTT TGT CCC 3' and PerkITS‐750 reverse 5 'ACA TCA CTT GGC CTA ATG ATG 3'.The extraction and amplification reagent used Promega Kit. Results of PCR showed the presence of Perkinsus sp.in blood cockle and blood clam from TPI Muara Angke, TPI Muara Kamal, TPI Muara Baru and TPI Cilincing on the 703 bp of electroforesis product. The highest prevalence of Perkinsus sp. in blood clam (Anadara inflata) located in TPI Muara Angke are 46 % followed by TPI Muara Kamal 34 %, TPI Muara Baru 34 % and TPI Cilincing 32 %. The highest prevalence of Perkinsus sp. in blood cockle (Anadara granosa) located in TPI Muara Kamal are 50 % followedby TPI Muara Angke 43 % , TPI Cilincing 32 % and TPI Muara Baru 26 %.
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